Computer-Aided Discovery ofTrypanosoma bruceiRNA-Editing Terminal Uridylyl Transferase 2 Inhibitors
نویسندگان
چکیده
منابع مشابه
Poliovirus replicase stimulation by terminal uridylyl transferase.
In an in vitro poliovirus replication system, purified viral polymerase, plus sense virion RNA, and a host factor have been previously shown to be necessary for the transcription of minus strands. We have found that a partially purified eukaryotic initiation factor-2 (eIF-2) fraction from rabbit reticulocytes can replace HeLa host factor in the replicase reaction. This enzyme preparation contai...
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A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg(2+). TUTase is present in vivo in at least two stable configurations: one contains a approximately 500 kDa TUTase oligomer and the other a approximately 700 kD...
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The catalytic, RNA-binding and oligomerization domains of the RNA-editing terminal uridylyl transferase 1 (RET1) from Leishmania tarentolae mitochondria were characterized by mutational analysis. Significant N- and C-terminal portions of the protein were found to be dispensable for UTP polymerization in vitro. Changes of conserved amino acids in the active site demonstrated a general similarity...
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G ene expression in the mitochondria of kinetoplastid protists like the human pathogen Trypanosoma brucei occurs via a remarkable pathway involving both the deletion of encoded uridines (Us) and the insertion of extra Us at defined positions within mRNAs synthesized from ‘‘cryptogenes’’ in the maxicircle DNA (see refs. 1 and 2 for recent reviews). In some cases, 50% of the nucleotides present i...
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Bilingual dictionaries present special difficulties for the lexicographer who is determined to employ the computer to facilitate his editorial work. In a sense, these dictionaries include evdrything contained in a normal monolingual edition and a good deal more. The singlelanguage definition dictionary is consulted as the authority on orthography, pronunciation and stress-pattern, grammar, leve...
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ژورنال
عنوان ژورنال: Chemical Biology & Drug Design
سال: 2014
ISSN: 1747-0277
DOI: 10.1111/cbdd.12302